PCR protocols, troubleshooting and research tools

Solutions and Buffers
Google
Web www.PCR-online.org

 

1. PubMed: a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's.

2. EMBO: The European Molecular Biology Organization

3. Nucleotides: The Entrez Nucleotides database is a collection of sequences from several sources, including GenBank, RefSeq, and PDB.

_____________________

 

 
 

Here are some commonly used PCR reagents and solutions:

10X TBE
1.5 L ddH2O
55 g Boric Acid
324 g Tris
18.6 g of Na2EDTA
Stir and fill to 2 L
Filter using Whatman #1

10X Loading Buffer
Add 10 ul stock bromophenol blue (0.1%) to 1 mL 30% Ficoll in TE

10X Taq Buffer (KCL/MgCL2)
100mM Tris-HCl - pH 8.8

500mM KCl,
0.8% Igepal CA-630,
15mM MgCl2.

 

   

 

 

 

 

   
 

 

 

 
 
 
 
   
© 2006 PCR-online.org