1. BLAST: BLAST® (Basic Local Alignment Search Tool) is a set of similarity search programs designed to explore all of the available sequence databases regardless of whether the query is protein or DNA.
2. Entrez Gene: A Directory of Genes.
3. The Quantitative PCR Primer Database : (QPPD) provides information about primers and probes that can be used to quantitate human and mouse mRNA by reverse transcription polymerase chain reaction (RT–PCR) assays.
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The Polymerase Chain Reaction, like most laboratory and experimental methods, can yield consistent and reproducible results. As with other protocols, some optimization is often necessary to adapt the process to a specific situation (species, fragment length, primer characteristics.)
Below are a collection of PCR protocols and tips to help obtain successful PCR results.
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